Concentrated
bovine colostral whey proteins from Streptococcus mutans/Strep. sobrinus
immunized cows inhibit the adherence of Strep. mutans and promote
the aggregation of mutans streptococci.
Loimaranta V,
Carlen A, Olsson J, Tenovuo J, Syvaoja EL, Korhonen H.
Institute of
Dentistry, University of Turku, Finland.
The aim of this
study was to examine the effect of bovine colostral whey proteins
from cows immunized with Streptococcus mutans/Strep. sobrinus on the
adherence and aggregation of caries-inducing bacteria, i.e., mutants
streptococci. Both adherence and aggregation are important phenomena
in the bacterial colonization of the human oral cavity. In all adherence
experiments there was a significant difference between treatments
by immune product (IP; from immunized cows) and a control product
(CP; a similar product from non-immunized cows). The adherence of
35S-labelled Strep. mutans cells (serotype c) to parotid saliva-coated
hydroxyapatite (SHA) was dose-dependently inhibited by both IP and
CP if SHA was coated with either product before exposure to bacteria,
but markedly lower concentrations of IP than CP were effective. When
instead of SHA the bacterial cells were pretreated with IP or CP,
only IP strongly and dose-dependently inhibited streptococcal adherence.
When bacteria, IP or CP, and SHA were incubated simultaneously, a
significant difference between IP and CP treatments was again found.
Further, IP effectively aggregated both Strep. mutans and Strep. sobrinus
cells, whereas hardly any effect was seen with CP. Both IP and CP
aggregated the control bacterium Strep. sanguis, which affected the
adherence of the pretreated bacteria.
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